Comparision of Packed Cell Volume by Manual and Automated Methods

Determination of packed cell volume by centrifugation.

Cell viability analysis using trypan blue: manual and automated methods.

One of the traditional methods of cell viability analysis is the use of trypan blue dye exclusion staining. This technique has been the standard methodology used in academic research laboratories and industrial biotechnology plants. Cells were routinely counted manually with a hemocytometer. In recent years, modern automated instrumentation has been introduced to supplement this traditional tec...

A comparison of manual and automated methods of measuring airway resistance and thoracic gas volume.

An automated method of measuring airways resistance (Raw) and thoracic gas volume (Vtg) by whole body plethysmography using a digital computer is described. The results obtained by this method are compared with those obtained by an experienced observer and the results of consistency tests on the two methods are given. There was wide variability in the values of Raw obtained using each method, a...

Validation of three viable-cell counting methods: Manual, semi-automated, and automated

A viable cell count is essential to evaluate the kinetics of cell growth. Since the hemocytometer was first used for counting blood cells, several variants of the methodology have been developed towards reducing the time of analysis and improving accuracy through automation of both sample preparation and counting. The successful implementation of automated techniques relies in the adjustment of...

Comparison of manual & automated analysis methods for corneal endothelial cell density measurements by specular microscopy.

PURPOSE To determine the reliability of corneal endothelial cell density (ECD) obtained by automated specular microscopy versus that of validated manual methods and factors that predict such reliability. METHODS Sharp central images from 94 control and 106 glaucomatous eyes were captured with Konan specular microscope NSP-9900. All images were analyzed by trained graders using Konan CellChek ...